An artificial virus model system with defined surface properties based on colloidal gold nanoparticles is introduced in this work. The engineered artificial virus nanoparticles comprise a monosialodihexosylganglioside (GM3) containing liposome wrapped around an 80nm Au nanosphere. Upon challenging Hela-CD169 cells, the artificial virus nanoparticles trigger a GM3-CD169-interaction-specific particle translocation on cell surfaces. The specific nature of the artificial virus nanoparticle that comprises a soft shell and a hard core results in virus-like behavior in which the particles are redistributed to confined spots in peripheral cell areas. The plasmonic core facilitates the continuous optical tracking of the artificial virus particle with extraordinary brightness and high temporal and spatial resolution in live cell imaging. The engineerable membrane provides a versatile platform to control the surface charge, lipid composition, and ligand density. Optimized artificial virus nanoparticles enable systematic investigations of virus infection pathways under defined conditions.

Colloidal artificial virus nanoparticle model system for characterizing ganglioside mediated virus infection

246th National Meeting (2013)

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