Self-assembled structural DNA nanostructures1 provide one of the most powerful routes to organize matter with very high resolution.  Although there are many applications for structures on the order of 100 nm in diameter2, in the nanoelectronics or optoelectronics domains, manufacture of much larger architectures will almost certainly be necessary. DNA origami structures can be catenated, enabling the growth of large structures by attaching programmable sticky end staples to opposite edges3.  However growing large addressable structures in two dimensions has proven quite challenging. This paper reports studies of the influence of programmable sticky end design on the kinetics of assembly and yield, as characterized using real-time PCR and atomic force microscopy. This study will help guide the development of sequential self-assembly, which is necessary to connect bottom-up methods of patterning with top-down approaches.  Fig. 1: 1D cross shaped origami prepared from short sticky end staples; scale bar 500 nm References:(1)  Seeman, N. C. Mol Biotechnol 2007 , 37, 246.(2)  Zhao, Y. X.; Shaw, A.; Zeng, X.; Benson, E.; Nystrom, A. M.; Hogberg, B. ACS Nano 2012 , 6, 8684.(3)  Mangalum, A.; Rahman, M.; Norton, M. L. J Am Chem Soc 2013 , 135, 2451.

Probing large DNA nanostructure self-assembly

246th National Meeting (2013)

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