We tested the effects of ellagic acid, urolithins A and B on pre-adipocyte differentiation, adipogenesis and inflammation in fat cells. Results indicated that ellagic acid and urolithin A inhibit adipogenesis (fat accumulation) but not differentiation. Urolithin B did not show that same effect. Modulation of fat accumulation seems to be through gene regulation of Glut 4. We tested the effects of ellagic acid, urolithins A and B on adipocytes challenged with lipopolysaccharide (LPS) to trigger inflammation. Results indicated that urolithins A and B reduce levels of nuclear phosphorilated NFkB. Gene expression of cytokines and proteins associated to the inflammation process indicate that urolithin A inhibit TNFa and iNOS, urolithin B inhibits only iNOS and ellagic acid inhibits TNFa, IL-6, iNOS and MCP-1. None of these compounds inhibited the gene expression of COX-2. The mode of action by which ellagic acid and urolithins A and B exert their anti-inflammatory properties will be discussed based on antioxidant (AOX) and AOX-independent mechanisms.

Modulation of adipogenesis and inflammation in adipocytes by ellagic acid and metabolites urolithins A and B

246th National Meeting (2013)

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