Protein-bound carbonyls have been shown to increase with age as well as in numerous diseases including rheumatoid arthritis, adult respiratory syndrome pulmonary fibrosis, diabetes, Parkinson's disease and Alzheimer's just to mention a few. The effect of the flavonoids quercetin and genistein were investigated according to their ability to inhibit the oxidation of human myoglobin and hemoglobin via the Fenton's pathway. Antioxidative activity of the flavonoids were determined by oxidizing myoglobin and hemoglobin in separate experiments with 20 �M Fe2+ and 0.1 mM hydrogen peroxide (H2O2) with and without quercetin or genistein. The samples were treated with quercetin or genistein at the concentrations of 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 �M respectively, dissolved in dimethyl sulfoxide (DMSO) and were incubated in a water bath at 37�C for 24, 12, and 8 hr. The levels of carbonylation were assayed by the protein carbonyl assay and the carbonyl levels were quantified and expressed per mg of protein. The results indicate that protein carbonyls for samples treated with quercetin or genistein decreased in a dose- dependent manner compared to the controls.The results indicate that quercetin compared to genestin at very low doses is more efficient in reducing the levels of protein carbonylation in hemoglobin and myoglobin.

Effects of quercetin and genistein on carbonylation in human hemoglobin and myoglobin

246th National Meeting (2013)

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