Recent advances in molecular science and nanotechnology offer unprecedented opportunities to miniaturize chemical analysis systems into nanofluidic devices that mimic ion channels and have single-molecule sensitivity, target-specific selectivity, and reduced consumption of materials [1]. Because the sequence-specific and label-free detection of DNA targets associates with many crucial pathogenic diseases [2], these nanofluidic devices have been endowed with chemical selectivity by integration with nucleic-acid-based sensing elements [3]. We showed an improvement nanofluidic sensor that can detect both nucleic acid and small molecule within one sensing platform. We achieved the detection limit of 10 fM for oligonucleotides and 1 nM for ATP. Particularly, these nanopore sensors could also be used in complex matrices with a high concentration of interfering substances in the buffer solution or even be directly used in serum. Figure 1. <img src='https://acs-archive-assets2.s3.amazonaws.com/239nm/uploads/2855_schmiedel_figure_1.jpg' alt='' />

Two-way nanopore sensing of sequence-specific oligonucleotides and small-molecule targets in complex matrices using integrated DNA super sandwich structures

246th National Meeting (2013)

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