We have recently synthesized brush-shaped, peptide-based copolymers by RAFT polymerization of peptide monomers with HPMA.  These materials possess the biological functions contributed by their peptide components with the scalable synthesis of synthetic polymers.   In this presentation, I will summarize our work in bioactive peptide identification, synthesis of peptide-based polymers, and applications of these materials for drug delivery.   In one example, we use a cell-based phage screening method to identify a peptide that preferentially binds to �anti-inflammatory� (M2) macrophage.  We further demonstrate that this peptide recognizes tumor-associated, M2-like macrophage, and that the peptide can be used for drug delivery to these cells.  In a second example, we synthesize multifunctional materials for neuron-targeted delivery of nucleic acids.  These peptide-based copolymers contain motifs for nucleic acid packaging, neuron targeting, and endosomal release.  We also synthesized enzymatically-cleavable polymers using peptide monomers containing cathepsin B-cleavable sequences.  The polymers were tested in vitro by reporter gene delivery to neuron-like, differentiated PC-12 cells and by direct, intraventricular injection in mice.

Phage panning, peptides, and polymers: From ligand identification to in vivo applications for drug delivery

246th National Meeting (2013)

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