AlphaScreen and AlphaLISA are fast and simple homogeneous immunoassay technologies allowing one to detect a wide range of analytes, either small (e.g., nucleotides) or very large (e.g., Titin = 4000 kDa ), interacting with high (fM) or low (mM) affinities. The versatility of the Alpha technologies is due to a unique energy transfer process based on singlet oxygen diffusion occurring between nano-sized polystyrene beads over a distance ranging up to 200 nm, making possible the development of assays involving a wide diversity of binding partners engaging simple or complex interactions. AlphaScreen and AlphaLISA are used as enabling tools for developing applications with biological targets often intractable due their size, complexity, or scarcity. Various customer study cases will be presented and discussed with the audience.

Characterizing intractable targets using homogenous immunoassays

246th National Meeting (2013)

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