A monoclonal antibody (designated as MAb 2B4C7E) which can recognize not only native but also heat-denatured Bt Cry1Ac protein was obtained. A sandwich ELISA was developed using the MAb 2B4C7E and rabbit polyclonal antibody to heat denatured protein. The detection limits for heat denatured and native Bt Cry1Ac protein was 30.5 and 0.5 ng/mL, respectively. The assay was able to detect heat-denatured Bt Cry1Ac protein extracted from boiled transgenic Bt corn which was undetectable with commercial Bt Cry1Ac ELISA Kit. The monoclonal antibody can also be used in Western Blotting of transgenic cotton and maize that contain Bt Cry1Ac gene.

Development of a sandwich ELISA for heat denatured Bt Cry1Ac protein

246th National Meeting (2013)

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