MitoNEET is a recently discovered mitochondrial [2Fe-2S] protein that is a binding partner of the anti-diabetic drug pioglitazone. MitoNEET contains a unique three cysteines and one histidine ligation of the metal cluster. However, the cellular function of mitoNEET is currently unknown. Several functions have been proposed including a role in cellular respiration, as an iron-sulfur cluster transfer protein, and as an electron-transport protein. Since each of these functions requires a binding partner, pull-down assays using histidine-tagged mitoNEET and cellular lysates from either mouse liver and HepG2 cells were done to capture and identify protein binding partners of mitoNEET. Proteomic analysis identified 25 and 22 proteins from two bands measuring approximately 70 and 72 kDa from the mouse and human liver cell lysates respectively. These putative protein-binding partners of mitoNEET were analyzed for cellular localization, cofactors, and function in order to elucidate the function of mitoNEET.

Characterizing protein binding partners of MitoNEET

246th National Meeting (2013)

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