We have developed an inexpensive disposable liquid culture bottle that detects and identifies opportunistic human pathogenic microorganisms at clinically relevant concentrations in liquid media during cell culture. The device combines amplification of bacteria with detection and identification in a single sealed bottle. This single step device is composed of a non-sterile chemical sensing array that is strategically interfaced with a culture bottle, without compromising the sterility of the device. Extensive results from nine microorganisms, including two strains of E. coli and two strains of E. faecalis, are reported illustrating the high reproducibility and identification ability of this technology.   The results indicate that this technology may be used to streamline the diagnosis of sepsis by eliminating the lengthy antibiotic susceptibility process.

Streamlining the diagnosis of blood stream infections by combining detection with identification

246th National Meeting (2013)

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