Coumestrol is a common phytoestrogen found in soybean seed.  A new procedure for analysis of soybean seed utilizing ultra performance liquid chromatography with tandem mass spectrometry detection (UPLC-MS/MS) was developed and validated.  The UPLC column selected for the assay, a 1.7�m, 2.1 x 100 mm Ethylene Bridged Hybrid [BEH] C18, improved resolution, sensitivity and speed.  The method procedure provided a UPLC run time of only 3/5 minutes per injection.  The UPLC-MS/MS assay precision was 13% (relative standard deviation).  Spiking recovery values ranged from 72-120%.  The method limit of quantitation (LOQ) was 0.1 ppm and the limit of detection (LOD) was 0.03 ppm.

Quantitative determination of coumestrol in soybean seed by ultra performance liquid chromatography-tandem mass spectrometry

246th National Meeting (2013)

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