TiO2 is known as the most widely used nanoscale, metal oxide. TiO2 nanoparticles are used in a variety of foods, toothpastes, sunscreens, cosmetics coatings, and pigments. Though nano-TiO2 has previously been found to have negligible toxic effects, studies have shown that nano-TiO2 generates reactive oxygen species (ROS) and cause inflammatory effects. In order to further understand the toxic effects, the European Committee for Standardization (CEN) uses the ISO 8692:2012 test organism, Pseudokirchneriella subcapitata, as a standard for water quality. The Pseudokirchneriella subcapitata alga has been dosed with the EC50 and the nano-TiO2 size established in the previous literature.            Changes in cell signaling, through analysis of signaling changes via differential protein phosphorylation to uncover molecular level changes occurring in Pseudokirchneriella subcapitata in the presence of the literature value EC50 of nano�TiO2. The proteins have been extracted and then separated into fractions using size exclusion chromatography by detecting 31P+. A series of separations of each fraction have been conducted for the phosphoproteins, then the phosphoproteins are enzymatically digested into phosphorylated peptides, selectively enriched, and followed by nanoLC-ESIMS for PMF.

Enhanced molecular level understanding of nano�TiO<sub>2 </sub>toxicity through phosphorylated protein identification

246th National Meeting (2013)

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Enhanced molecular level understanding of nano�TiO2 toxicity through phosphorylated protein identification

Enhanced molecular level understanding of nano�TiO₂toxicity through phosphorylated protein identification