Mast cells are granulated leukocytes known to play a role in type I hypersensitivity reactions and other inflammatory diseases. Upon activation, mast cells release various chemokines and cytokines. Mast cells' most often considered mode of activation is through IgE-mediated response via the FceR1 receptor, which senses antigen in the environment. Surprisingly, both the asthma-relevant cytokines CXCL10 and RANTES have been found to stimulate mast cell mediator secretion directly through a potentially different pathway. In this work, we examined secreted mediators from murine mast cells with high performance liquid chromatography with electrochemical detection, ultraperformance liquid chromatography coupled to a triple quadrupole mass spectrometer, and single cell carbon fiber microelectrode amperometry.  Our results have shown time- and concentration- dependence of mediator secretion upon chemokine exposure both in ensemble and single cell analysis.

Bulk and single mast cell degranulation upon exposure to the chemokines CXCL10 and RANTES

246th National Meeting (2013)

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