The identification of potential cell surface targets used by host- or pathogen-associated sugars for infection has been challenging. Herein we report the use of new photo-activated covalent cross-linking agents designed for facile attachment of carbohydrates for the study of carbohydrate-protein interactions with a specific focus on synthetic Caenorhabditis elegan-associated saccharides. Challenges in identifying the protein targets using mass spectrometry methods with adequate signal to noise levels will be discussed.

Study of carbohydrate-protein interactions using new cross-linking agents and target identification through mass spectrometry

246th National Meeting (2013)

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