Fundamental characteristics of Lauryl Acrylate Porous Polymer Monoliths (PPMs) were investigated using High Pressure Liquid Chromatography (HPLC). Previous studies utilized Capillary Electrochromatography (CEC) to successfully measure the thermodynamic characteristics of these PPMs. However, CEC uses electroosmotic flow (EOF) to transport the mobile phase through the column, leaving the experiments without an accurate volume measurement. Volumetric properties of the mobile and stationary phases of the column require a more precise value of the flow rate to measure these properties. A nano-flow HPLC was used to measure the column efficiency properties, including porosity and phase ratio of these columns. Porosity is a measurement of the void spaces in a material; therefore it is used to quantify the free space in our PPMs.  The total, external, and internal porosities were measured in the presence and absence of EOF modifiers. Our data supports that despite the presence of these organic EOF modifiers, the internal porosity of our PPMs was found to be significantly less than traditional monoliths found in the literature.  The phase ratio is the ratio of the volume of stationary phase to the volume of mobile phase in the column and was found to be 0.189 +/- 0.002 on a typical Lauryl Acrylate PPM column. With a nano-flow HPLC, the properties of Lauryl Actylate Porous Polymer Monoliths are characterized and found to be different from traditional stationary phases.

Characterizing Lauryl acrylate porous polymer monoliths using HPLC

246th National Meeting (2013)

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