Analysis of microRNAs (miRNAs) has attracted tremendous research interest. We here constructed a 3-D stem-loop miRNA biosensor by connecting a hairpin probe onto a tetrahedron DNA nanostructure with 3 sulfur. The DNA nanostructure improved the spatial control and accessibility of the probes. As demonstrated in Figure 1. Initially, the stem-loop probe was in �closed� state, hindering the end of the probe from being approached by the signal probes (SP). Target hybridization would break the stem duplex and exposed the probes to the SPs. Then an avidin-HRP was captured and lead to a significantly catalyzed current signal. Analysis results of miR-141 are showed in figure 2.Figure 1 , Scheme of the 3-D stem-loop biosensor for miRNA analysis.Figure 2 , A) CV curves of the 3-D stem-loop biosensor with blank, 10 fM and 1nM target miRNA (miR-141). Scan rate: 100 mV/s.; B) I-t curves for detection of miR-141 with concentrations of 0, 10 aM,10 fM,10 pM,100 pM and 1 nM.

Highly sensitive miRNA analysis using stem-loop probes carried by tetrahedron DNA nanostructures

246th National Meeting (2013)

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