In this study, the proof-of-principle application of the newly developed Metal Assisted � Microwave Accelerated Evaporative Crystallization (MA-MAEC) technique to the crystallization of lysozyme is demonstrated. Polyethylene glycol monomethyl ether was used as the solvent of choice for both room temperature crystallization and the MA-MAEC technique. At room temperature, complete crystallization of lysozyme occurred at 1,560 minutes with crystal sizes ranging between 15-440 �m. Using the MA-MAEC technique, complete crystallization of lysozyme was achieved at 555 minutes, and the same defined hexagonal crystal morphology as that of lysozyme crystallized at room temperature was observed. Crystal sizes using this technique ranged between 41-1630 �m. MA-MAEC was demonstrated to be a more effective technique in the rapid crystallization of macromolecules in comparison to room temperature crystallization

Crystallization of lysozyme using metal-assisted and microwave-accelerated evaporative crystallization

247th National Meeting (2014)

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