In recent years, production of tangerines (Citrus tangerina) has rapidly increased due to their taste, bright-orange color, and being easy to peel. Apart from their sensory attributes, they also contain a wide array of health benefiting compounds. In particular, limonoids have been investigated for antibacterial, antifungal, antiviral, and anticarcinogenic activity. In order to provide proof of concept, limonoids need to be tested in animal and human intervention trials. To facilitate the progress of in vivo experiments, purification methods to obtain high purity compounds are needed. In the present study, we have extracted tangerines with ethyl acetate and purified by flash chromatography. The compounds were detected at 210 nm and 280 nm. Fractions were pooled based on analysis by HPLC to obtain four compounds and their structures were confirmed by high resolution LC-MS. To the best of our knowledge, this is the first study on purification of bioactive compounds from tangerines.

Isolation of bioactive compounds from Dancy tangerine using hyphenated liquid chromatography with photodiode array detection

247th National Meeting (2014)

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