Hemicelluloses are abundant, biodegradable, nontoxic raw materials that can be used in different applications as an alternative to non-renewable fossil resources. In this work, spruce xylan was utilized to obtain in situ forming hydrogel as suitable extracellular matrices for tissue engineering needs. In situforming hydrogels are injectable (have minimal invasive damage) and allow homogeneous distribution of bioactive molecules within the surrounding tissue.  An in situ forming spruce xylan-based hydrogel was synthesized through the consecutive steps of bioconjugation between carboxylic groups present in glucuronic acid residues of spruce xylan and tyramine with subsequent enzymatic crosslinking via decomposition of hydrogen peroxide. Average time of gelation was about 20 seconds. Rheological analysis confirmed instant crosslinking of the material and showed viscoelastic phenomena during gelation time. The hydrogel was successfully tested for the cell immobilization of mesenchymal stem cells. It provided attachment and subsequent proliferation and differentiation for the encapsulated cells. From all the above we conclude that hydrogels made of spruce xylan are promising materials for tissue engineering purposes.

<i>In situ</i> injectable hydrogels based on spruce xylans for drug and cell delivery

247th National Meeting (2014)

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In situ injectable hydrogels based on spruce xylans for drug and cell delivery