Quantitative proteomics offers several approaches for the relative quantitation of proteins which includes label-free analyses, isotopic labeling strategies, and the incorporation of isobaric tags.  Recently, we introduced an approach that combines precursor isotopic labeling and isobaric tagging, also known as 'cPILOT'.  This strategy takes advantage of various chemistries to tag N-terminal or specific amino acid sites with a precursor isotope tag or with a commercially available reagent such as iTRAQ or TMT.  The primary advantage of cPILOT is increased sample multiplexing.  Additionally, MS3 methods have been developed in order to improve reporter ion quantitation without compromising the number of identifiable and quantifiable spectra.  An overview of these novel cPILOT strategies will be presented as well as preliminary results from proteomic analysis of mouse tissues.

Combined precursor isotopic and isobaric tagging (cPILOT) proteomics strategies

247th National Meeting (2014)

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