Novel cationic sulfonyl chloride derivatizing reagents were synthesized from commercially available zwitterionic surfactants and evaluated in LC-ESI-MS/MS experiments.  By preparing reagents of varying hydrophobicity, retention of derivatized analytes on reversed phase columns is adjusted, granting the ability to minimize signal suppression due to co-eluting matrices.  Signal enhancements as large as 300% were observed by increasing the alkyl chain length of the derivatizing reagent.  Excellent sensitivity, linearity and reproducibility are observed in LC-MS/MS methods.  A detection limit of 25 fg/mL is reported for derivatized p-cresol and S/N > 400 is observed for derivatized �-estradiol extracted from an aqueous matrix at a concentration of 10 ng/mL.  Detection limits for derivatized phenols extracted from various biological matricies is presented illustrating the applicability for trace bioanalytical quantitation of small molecules that show poor inherent signal intensity in LC-MS/MS methods.

New derivatization reagents to optimize retention and response for quantitative analysis by LC-ESI-MS/MS

247th National Meeting (2014)

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