Lipopolysaccharides (LPS) are major virulence determinant in Gram-negative bacteria, which are responsible for many pathophysiological responses to bacterial infections and can elicit strong immune responses in animals. LPS typically consist of three main parts: an endotoxic lipid A component with a bisphosphorylated and acylated �-(1?6)-inter-linked glucosamine disaccharide backbone, a nonrepeating core oligosaccharide, and a distal polysaccharide named as O-antigen with O-antigenic component specific to each bacterial species. The research on LPS has attracted much interest for the development of vaccine candidates, diagnostic tools and therapeutics. Many lipid A and its analogs have been found as a potent non-toxic vaccine adjuvant. In order to better understand the role of LPS in host-pathogen interactions and elucidate the antigenic and immunogenic properties of LPS inner core region. Based on biosynthetic pathway of LPS, chemo-enzymatic synthesis of inner core oligosaccharides of E.coli was performed in a sequential enzymatic reactions using chemically synthetic lipid A as substrate. Synthesis of these well-defined oligosaccharides can also provide various substructures for establishing structure-activity relationships for determining minimal epitope which can elicit protective immune response. Therefore, chemo-enzymatically constructed pure E. coli inner core-lipid A conjugates would represent as a novel set of wide-spectrum and stand-alone vaccine candidates.

Chemo-enzymatic synthesis of inner core oligosaccharides of<i> E. coli</i>

247th National Meeting (2014)

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Chemo-enzymatic synthesis of inner core oligosaccharides of E. coli