Many methods have been used in the encapsulation or immobilization of enzymes. One of them is the sol-gel process which can be used to produce porous silica materials even at ambient temperature. In this study, several enzymes, namely catalase, lipase, laccase and glucose oxidase were encapsulated by sol-gel process using tetramethyl orthosilicate (TMOS) as alkoxide. The behavior of the enzymes encapsulated within gel matrix was monitored by different spectroscopic methods to determine the integrity of enzymes. The results were compared with the enzymes in solution form. Although there are minor difference between the spectra in different matrices (solution and gel), the integrity of the enzymes were found to be maintained when they are immobilized within the gel matrix.

Encapsulation of enzymes using the sol-gel process

247th National Meeting (2014)

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