Protein powders are easily acquired in today's market. Cloud point extraction was used to concentrate lead in solution using Triton X-114 and 1-phenylthiosemicarbazide (1-PTSC). The conditions for formation and isolation of the lead-containing micelles included adjusting the pH to 9.0 prior to addition of the detergent and 1-PTSC, and heating the samples. The metal-rich phase was isolated by centrifugation and then cooling. The phase containing the concentrated metals was suspended in nitric acid/methanol mixture prior to analysis. This method was used first with a series of lead standards and then later with digested protein powder samples. All samples were analyzed on a Perkin-Elmer 400 AAnalyst atomic absorption spectrometer.

Preconcentration of lead in digested protein powders for analysis in flame atomic absorption spectrometry

247th National Meeting (2014)

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