Hydrogel containing phenylbronic acid (APB  ) evinced good sensitivity and selectivity to glucose molecules. 3-APB moieties in hydrogel perform a role as a reversible cross-link, forming dual binding structures with cis-diol of glucose molecules, but only producing single binding structures with fructose molecules. However, there are still several problems should be solved such as sensitivity, and response time. Scaling down to micro size, and confirming of the relevance of chemicals are necessary to develop function of glucose sensitive hydrogel (GSH  ). In this research, design of experiment (DOE ) was used for getting reliable results to confirm of chemical composition effect on GSH with minimum samples. DOE has been used for industrial field to enhance reliability and yield of products within limited cost and time. For this study, DOE was run by 3 factors with continuous level. And, two types of responses such as magnitude and 1st order rate were used for analysis. Regression analysis showed that all factors affected response magnitude and 1st order rate of GSH (Table 1, meaningful P-value < 0.05). However, 2nd order interaction between APB/DMAPAA * wt%, and AAm/BIS *wt% were shown as main effect factors for response magnitude of GSH. The first order rate was mostly affected by AAm/BIS.Table 1. Effectiveness Evaluation of Parameters  Surface analysis of experiment results showed that the sensitivity of GSH increased as increasing of the molar ratio of 3-APB/DMAPAA and wt% of pregel solution (figure 1.a). Faster 1st order rate was achieved with less BIS molar ratio (figure 1.b). ?Figure 1. Surface plots of GSH responses to glucose concentration changes (1mM to 5mM in 1X PBS), (a) Magnitude vs. APB/DMAPAA & wt%, (b) 1st order rate vs. AAm/BIS & wt%. (dot: actual data points) From this study, the relevance of chemical components what a GSH is consisting of was confirmed by DOE. It is believed that this study can help for enhancing of a GSH's function and devising of an invasive glucose sensing system with the hydrogel for medical application in the future.

Effect of composition changes on sensitivity of glucose sensitive hydrogel studied by design of experiments (DOE)

247th National Meeting (2014)

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