In this study, silica nanoparticles, fabricated by the St�ber method, were transformed into multifunctional nanoreactors for use in biosensing applications. Bare silica nanoparticles were modified with 3-aminopropyl trimethoxysilane (APTMS) to allow covalent attachment of the luminescent dye, Pd(II) meso-tetra-4-carboxyphenyl porphine (PdP), with EDC and sulfo-NHS. An enzymatically-active region was introduced through layer-by-layer self-assembly (LbL) using poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) to immobilize the enzyme glucose oxidase (GOx) on the particles (Figure 1). Figure 1. <img src='https://acs-archive-assets2.s3.amazonaws.com/240nm/uploads/39707_figure1.jpg' alt='' /> The particles retained activity after being immobilized in a poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogel and tested for optical response to glucose under physiological conditions (Figure 2). Results demonstrate: 1) silica nanoparticles can be used as templates for immobilizing sensing chemistry to create independently-functioning nanoreactors and 2) these nanoreactors can be incorporated into larger implantable hydrogel structures for in vivo sensing applications.

Fabrication and characterization of optically-responsive glucose sensing nanoreactors

247th National Meeting (2014)

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