Rachel Unruh
Michael Mcshane
In this study, silica nanoparticles, fabricated by the St�ber method, were transformed into multifunctional nanoreactors for use in biosensing applications. Bare silica nanoparticles were modified with 3-aminopropyl trimethoxysilane (APTMS) to allow covalent attachment of the luminescent dye, Pd(II) meso-tetra-4-carboxyphenyl porphine (PdP), with EDC and sulfo-NHS. An enzymatically-active region was introduced through layer-by-layer self-assembly (LbL) using poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) to immobilize the enzyme glucose oxidase (GOx) on the particles (Figure 1).
Figure 1. The particles retained activity after being immobilized in a poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogel and tested for optical response to glucose under physiological conditions (Figure 2). Results demonstrate: 1) silica nanoparticles can be used as templates for immobilizing sensing chemistry to create independently-functioning nanoreactors and 2) these nanoreactors can be incorporated into larger implantable hydrogel structures for in vivo sensing applications.
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