Surface properties are known to be one of the key factors in cell adhesion and proliferation because eukaryotic cells have to adhere through the extra-cellular matrix to function properly. Using a homogeneously mixed amine and thiol functional group on self-assembled monolayers (SAMs) anchored on glass, surfaces with a series of zeta potential from about +30 to -150 mV at pH 7.4 are obtained. Furthermore, it is found that amine and thiol SAMs on glass poses low cytotoxicity. Thus, this binary-SAM modified glass is ideal for studying the effect of surface potential on cell behaviors including adhesion and proliferation. The surface chemical composition of binary SAM is quantified using x-ray photoelectron spectroscopy (XPS) and the actual zeta potential of surfaces in physiological condition is measured with an electro-kinetic analyzer. NIH3T3 cells are chosen to culture on these binary-SAM modified glass and incubated for several different time period. The coverage and shape of cells adhered to surfaces of various potential are examined by fluorescence optical microscopy and scanning electron microscopy. It is expected that the positive surface potential promotes cell adhesion and proliferation until contact inhibition occurred.

Effect of surface potential on cell adhesion and proliferation

247th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.