Due to wide spread applications and commercializations of fullerene (C60) systems, both human and environmental systems become increasingly exposed to these materials. As a result there is concern of the biological and environmental effects of these systems. Even though fullerene has negligible solubility in water, the ability to form stable aqueous C60 colloids (known as nano-C60 or nC60) and the availability of these in natural systems at environmentally-relevant concentrations led to the studies of its toxicity. Previous studies have indicated that fullerene in colloidal form can induce oxidative stress in living systems. This research is focusing on the oxidation caused by fullerene colloidal suspensions. Stable aqueous fullerene colloidal suspensions are demonstrated to rely on the [6,6]-closed epoxide derivative of the fullerene (C60O) for stability and this derivative is present in small quantities in nearly all nC60 preparations due to a reaction with air. Presence of C60O in these colloidal suspensions considered to have increased ability of oxidation. For this work nC60 samples prepared by enrichment of C60O in the colloidal particles and regular nC60 preparations have been used as a comparative study to monitor oxidation. Fluorescent assays for monitoring oxidative behavior of these suspensions with the probe molecule dihydrorhodamine-123 (DHR123) as the indicator of oxidation have been investigated.

Dihydrorhodamine-123 as a sensitive indicator of oxidation caused by aqueous nano-C<sub>60</sub> suspensions

247th National Meeting (2014)

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Dihydrorhodamine-123 as a sensitive indicator of oxidation caused by aqueous nano-C60 suspensions

Dihydrorhodamine-123 as a sensitive indicator of oxidation caused by aqueous nano-C₆₀ suspensions