Escherichia coli, also known as E. coli, is a bacteria that may contain a serotype O157:H7, which causes food poisoning in humans and may become life-threatening. In order to counteract this occurrence, an enzyme inhibitor of E. coli needs to be discovered. We are researching nucleoside hydrolase, rihC, in E. coli, which is an enzyme that catalyzes the hydrolysis of a nucleoside to its base and ribose. This enzyme is present in E. coli and absent in humans. RihC is able to catalyze the hydrolysis of both purine and pyrimidine nucleosides. In order to implement this experiment, the enzyme has been cloned and purified to homogeneity. The kinetic parameters, kcat and Km, have been determined for a number of nucleosides. Further analysis will involve the determination of the kinetic mechanism and a study of structure activity relationship with the ultimate goal of developing an inhibitor of the enzyme.

Kinetics of nucleoside hydrolase, rihC, in <i>E. coli</i>

247th National Meeting (2014)

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Kinetics of nucleoside hydrolase, rihC, in E. coli