Mycoplasma suis is a blood-borne pathogen of pigs. Both chronic and acute forms of infection have been reported; while chronically infected animals show few clinical signs of disease, acute infection is characterized by fever, listlessness, anorexia, and in severe cases, by a life-threatening anemia. The detection of M. suis in blood samples using quantitative PCR (qPCR) depends on the efficiency of the extraction method used. This study evaluated three commercially available DNA extraction kits (Zymo Quick-gDNA� Blood MiniPrep, Qiagen DNeasy Blood &Tissue Kit; and Promega ReliaPrep� Blood gDNA Miniprep System) to determine the relative recovery of M. suis from pig blood. Serial dilutions of whole EDTA-blood from an M. suis infected pig were subjected to DNA extraction using the various kits as per the manufacuturer's instructions. Yield and purity of total genomic DNA extracted from the blood were compared spectrophotometrically while detection of the 16S rRNA gene of M. suis was performed using a species- specific Taqman qPCR assay specific for M.suis.

Comparison of three DNA extraction kits for detection of<i> Mycoplasma suis</i> DNA from blood as assessed by quantitative PCR

247th National Meeting (2014)

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Comparison of three DNA extraction kits for detection of Mycoplasma suis DNA from blood as assessed by quantitative PCR