A matrix-assisted laser desorption/ionization reflectron time-of-flight mass spectrometer (MALDI RTOF-MS) was rebuilt and calibrated. The potentials were modeled using SimION Ion and Electron Optics Simulator. Calibration standards were used to optimize the voltage potentials. The standards used were samples of C-60 fullerene, Bradykinin Fragment 1-7, ACTH Fragment 18-39, Angiotensin II, P14R, and Insulin chain B oxidized which provided a mass range between 720-3494 Da. The same samples were used to measure mass spectra and optimize signal via a HIMAS linear detector. The HIMAS detector is composed of a conversion dynode, single microchannel plate (MCP), scintillator, and photomultiplier tube (PMT). The data was collected and analyzed using National Instruments LabView software and a Tektronix DPO 3054 oscilloscope. The experiments were run multiple times to ensure reproducibility; and mass spectra obtained were compared to literature spectra to ensure accuracy.

Calibrating a HIMAS linear detector on a matrix-assisted laser desoprtion/ionization reflectron time-of-flight mass spectrometer

247th National Meeting (2014)

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