Methylglyoxal (MGO) is a highly reactive dicarbonyl compounds, accumulating in the patients plasma with diabetes and causing cell toxicity. In this paper, we studied the MGO toxicity on rat pheochromocytima cells in vitro. The apoptosis were detected by flow cytometric analysis. Cell morphology was observed by microscopy and mortality was calculated. The genome DNA and cell protein were studied by gel electrophoresis. The results showed that the mortality and apoptosis significantly increased than control after rat pheochromocytima cells were treated by MGO, and the electrophoresis characteristics of rat pheochromocytoma cells genome DNA was changed. Fluorescence spectrum analysis showed that MGO resulted in the decrease of fluorescence intensity of rat pheochromocytima cells protein. The intracellular ROS continued to increase after the cell incubated with MGO which may play an important role in cell injury. These cytotoxic action may be implicated in the development of diabetic complications.

Cytotoxic action of methylglyoxal in rat pheochromocytoma cells

247th National Meeting (2014)

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