To earn and maintain a share in the fast-growing peptide market, peptide researchers and manufacturers are constantly trying to improve and optimize the various steps in peptide synthesis. One of the main bottlenecks in peptide synthesis is the purification step. Techniques such as FPLC and preparative HPLC are limited by small loading amounts, long separation times, poor recoveries and high costs. In this work, we demonstrate that flash chromatography offers a rapid purification technique that improves recovery, yield and purity for a wide range of peptide. A new wide pore C18 phase expands flash purification capabilities to larger sized peptides, while providing better resolution. We present data to show the benefits of higher loading and faster purifications in peptide purification.

Rapid purification technique improves recovery, yield, and purity for a wide range of peptides using flash chromatography

247th National Meeting (2014)

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