We report an approach to activate inert hydrocarbon monolayers with UV light to fabricate DNA microarrays. Unlike like traditional microarrays which require reactive functional groups on the surface, our DNA microarray is built upon an inert layer of N,N-dimethyl-N-octadecyl(3-aminopropyl)trimethoxysilyl chloride silane (DMOAP). This layer is activated by UV (254 nm) just prior to the immobilization of oligonucleotides probes. Our XPS results show that new functional groups such as alcohol (C-O), aldehyde (C=O) and carboxylic acid (O-C=O) form on the surface after the UV exposure. Among them, aldehyde groups are responsible for the immobilization of amine-label oligonucleotides. By using this approach, we further optimize several factors affecting the immobilization of oligonucleotides to obtain a DNA microarray on which the density of oligonucleotides is up to 0.16 pmole/mm2.This DNA microarray is able to differentiate solventogenic Clostridium sp. such as Clostridium acetobutylicum, Clostridium butylicum, and Clostridium beijerinki.

DNA microarrays on UV-modified surfaces for differentiating clostridium species

247th National Meeting (2014)

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