The sensitive detection of cancer biomarkers in urine could revolutionize cancer diagnosis and treatment. Such detectors must be inexpensive, easy to interpret, and sensitive. This work describes a bioaffinity matrix of viruses integrated into PEDOT (poly-3,4-ethylenedioxythiophene) films for electrochemical sensing of prostate-specific membrane antigen (PSMA), a prostate cancer biomarker.High sensitivity to PSMA resulted from synergistic binding action of two different ligands to PSMA present on the same phage particle. The primary recognition ligand was genetically encoded, and the secondary recognition ligand was chemically synthesized to wrap around the phage surface. The dual ligands result in a bidentate binder with high copy, dense ligand display for enhanced PSMA detection through a chelate-based, avidity effect. Biosensing with virus-PEDOT films provides a 100 pM limit of detection for PSMA in synthetic urine without requiring enzymatic or other amplification.

Sub-nanomolar detection of prostate-specific membrane antigen in synthetic urine by synergistic, dual-ligand phage

247th National Meeting (2014)

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