The sensitive detection, quantification, and sequencing of highly negatively charged glycosaminoglycan still have been very challenging tasks for analytical chemists. Three major contributions to this topic from our group will be discussed in the talk. (1) Sensitivity detection:  Recently, our group has developed the ultrasensitive (zmol, 10-21 mol molecules) detection techniques for glycosaminoglycans based on Glyco-qPCR and capillary electrophoresis and a method relying on quantum dot-based fluorescence resonance energy transfer   . (2) Accurate quantification  : We have developed a LC-MS/MS based methodology targeting at various tissue and cell sample analysis. This LC-MS/MS method enables simultaneous separation and quantification of more than 20 disaccharides and 4 tetrasaccharides existing in glycosaminoglycans (CS/DS, HA and HS) with ftmol (10-21 mol molecules) level of detection sensitivity. Moreover, the sample preparation process has been simplified to achieve high throughput analysis. (3) Glycosaminoglycan sequencing:  We have developed a HILIC-FTMS and MS/MS platform to do on-line sequencing of the complex oligomer digested from intact glycosaminoglycan  .

New analytical methodologies used for glycosaminoglycan quantification and sequence analysis

247th National Meeting (2014)

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