There has been a quiet revolution in the ability to carry out programmed reactions based on strand exchange.  A variety of non-enzymatic DNA circuits have now been built that can carry out complex computations, including taking square roots and acting as neural networks.  We have explored a reaction class known as catalytic hairpin assembly (CHA), and shown that it can be used for amplification and signal transduction in a variety of analytical applications.  In particular, it has proven to be extremely useful as a means of monitoring enzyme-based isothermal amplification reactions.  By rationally modifying the basic design and chemistry of the CHA reaction, we have been able to increase signal, suppress noise, and modulate interactions with a variety of amplifiers and amplicons at a variety of temperatures.

Strand exchange reactions at the frontier: Modular reaction networks

247th National Meeting (2014)

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