Gold nanoparticles have many potential biomedical applications, but require specific cell targeting. This can be achieved by labeling proteins with streptavidin then crosslinking them to the nanoparticles. We will report on our work linking gold nanoparticles of various sizes and ligand shells to a green fluorescent, glutathione-S-transferase biotinylated fusion protein. The nanoparticle-protein system is analyzed by high performance liquid chromatography. Dynamic light scattering and UV-Vis spectroscopy of the conjugate is used to determine size and surface plasmon resonance position of the system. After inserting the gold nanoparticle protein conjugate into the HEK-293T cells, fluorescent electron microscopy is used to specify the location of the nanoparticles in the cells. The resulting system is analyzed in regard to conservation of the properties of the gold nanoparticles such as its surface plasmon resonance.

Crosslinking biotinylated proteins to gold nanoparticles and inserting them into HEK-293T cells

247th National Meeting (2014)

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