The polishing steps of monoclonal antibodies present several challenges. One of the major goals of the purification process is to reduce the amount of aggregates. Typically, cation exchange chromatography is included in the purification scheme to address this problem. This presentation will give an overview of a new grafting technology that enables attachment of polymeric surface extenders with cation exchange functionality onto agarose beads for use in downstream processing of monoclonal antibodies. The impact of the surface extenders design on chromatography media in terms of aggregate removal and dynamic binding capacity will be discussed. Furthermore, studies where the performance of existing products is compared to our prototypes will be presented.

Development of a new grafting technology for the production of cation exchange media for polishing of monoclonal antibodies

247th National Meeting (2014)

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