Cellulose is the most abundant biomass on the earth. Butanol production from lignocellulosic biomass by fermentation has been considered to have great economic and environmental benefits. C. cellulovorans is able to use cellulose directly, but not able to produce butanol. In this work, we characterized the restriction-modification system of C. cellulovorans and developed a novel transformation method to overcome its restriction barrier. By introducing the n-butanol biosynthesis pathway, we successfully engineered C. cellulovorans to produce n-butanol directly from cellulose. The fermentation process was optimized to enhance n-butanol production and reduce the acid byproducts, making the process attractive for industrial application. This paper will highlight the strategy applied in metabolic engineering of C. cellulovorans. The characterization and fermentation results from the mutant strain will also be presented.

Metabolic engineering of cellulolytic Clostridium cellulovorans for n-butanol production from lignocellulosic biomass

247th National Meeting (2014)

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