Zinc (Zn) is a well known important element involved in normal human physiology.  Also, metal Zn oxide nanoparticles  (ZnO NPs) could be made in order to induce a wide range of biological responses in cell systems. Doping ZnO NPs with transition metals like manganese (Mn) increases their surface defects. Therefore, this modification affects the optical and electronic properties of these NPs and could improve photo-oxidation reactions in photodynamic therapy (PDT). In this way, Mn-doped ZnO NPs could be designed and used to treat B-CLL, especially when combined with PDT.  If these NPs are excited by light emission from a green laser with a wavelength of around 532nm, intracellular generation of cytotoxic reactive oxygen species, especially singlet oxygen occurs leading to a possible therapeutic apoptotic effect that could kill B-CLL cells even those with resistant phenotypes. Normal lymphocytes as well as B-CLL cells were penetrated by Mn-doped ZnO NPs when cultured at 37o C for two hours and observed under TEM.  A significant production of intracellular singlet oxygen was detected in both normal and B-CLL cells after being penetrated by NPs and laser irradiated.0.5% Mn-doped ZnO NPs produce a high level of apoptosis on un-mutated B-CLL cells compared to normal lymphocytes, especially when they are laser irradiated. This significant difference in the apoptotic level between normal lymphocytes and leukemic cells could be related to the difference in the redox state usually higher in the malignant B-CLL cells. Resistant to traditional therapies un-mutated B-CLL cells responded strongly to the cytotoxic effect of Mn-doped ZnO NPs after PDT, suggesting that this innovative therapy with modifications could be used in the near future as an alternative effective treatment specially under the occurrence of B-CLL difficult to treat cell phenotypes.

Manganese-doped zinc oxide nanoparticles for the treatment of B-chronic lymphocytic leukemia by photodynamic therapy

247th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.