Synthetic peptides represent an increasingly important class of therapeutic agents. Peptide purification represents a major bottleneck in peptide discovery and development. Advances in peptide synthesis have resulted in significantly improved crude purities of even complex peptides. As a result, the goal of peptide purification is often to remove non-peptide impurities and does not require the time, resolution and effort required for semi-preparative scale HPLC.Here we report expedited purification methods for a variety of peptides over a broad range of scales utilizing mass-directed reversed-phase flash chromatography. Results demonstrate how transitioning from traditional HPLC purification methods to flash chromatography for peptide purification following synthesis can significantly impact peptide throughput and ease peptide discovery and development.

Mass-directed flash purification of peptides

247th National Meeting (2014)

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