Aptamers are small single-strand DNA or RNA oligonucleotides with a very high selectivity and affinity for their targets, which can be screened from synthetic random oligonucleotides library by SELEX (systematic evolution of ligands by exponential enrichment) in vitro. Selection of aptamers against HBsAg would contribute to the better detection and diagnosis of  hepatitis B virus infection. In this work, we first adopted SELEX method using carboxyl magnetic nanoparticles as matrix and prepared ssDNA library by Asymmetric PCR amplification for the next selection round. Binding affinity of aptamers with target HBsAg was determinated by ELOSA (Enzyme league oligonucleotide adsorption test) at 450 nm with microplate spectrophotometer. At last, in order to obtain aptamer sequences, the final selected products were purified by gel electrophoresis, then cloned and sequenced. In conclusion, DNA aptamers that recognize HBsAg specifically was successfully obtained via 13 rounds selection, and the secondary structures of aptamers were analyzed by DNAMAN software.

Selection of DNA aptamers against HBsAg using carboxyl magnetic nanoparticles as matrix by SELEX

247th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.