Conventional size-exclusion chromatography (SEC) and size-exclusion chromatography coupled to differential viscometry (SEC/DV) methodologies have been developed and applied to the dilute solution characterization of crosslinked polystyrene (PS) nanoparticles. A single SEC/DV experiment provides a wealth of information about PS nanoparticle structure including absolute molecular weight distribution, particle size distribution, volumetric swelling factor and solute conformation. Nanoparticle sizes determined by SEC/DV are in excellent agreement with those determined by other techniques such as hydrodynamic chromatography, multi-angle light scattering, dynamic light scattering and transmission electron microscopy. SEC/DV is applicable over a wide range of sizes from a few nanometers to several hundred nanometers. This talk will include discussion of SEC/DV methodology development for nanoparticle PS characterization. The approach for extracting size and volumetric swelling parameters from SEC/DV data will be presented. Solute conformation gleaned from Mark-Houwink plots of PS nanoparticle samples will also be presented. Finally, SEC/DV-based sizes will be compared to those obtained from other techniques.Figure 1 � Absolute molecular weight distributions and Mark � Houwink plots of large (red traces) and small (blue traces) crosslinked PS nanoparticle examples  

Characterization of crosslinked polystyrene nanoparticles by size-exclusion chromatography coupled to differential viscometry

247th National Meeting (2014)

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