The polishing steps of monoclonal antibodies present several challenges. One of the major goals of the purification process is to reduce the amount of aggregates. Typically, cation exchange chromatography is included in the purification scheme to address this problem.A new grafting technology has been developed, enabling attachment of polymeric surface extenders with cation exchange functionality onto agarose beads for use in polishing of monoclonal antibodies. The performance of these polymer grafted cation exchangers has been investigated and will be presented. The polishing step for different monoclonal antibodies was optimized regarding buffer composition and pH using 96-well filter plates and later confirmed in packed bed using column format. Yield, aggregate- and HCP- removal was monitored throughout the study.

Polishing of monoclonal antibodies using a polymer grafted cat-ion exchanger

247th National Meeting (2014)

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