Biosensors that convert recognition of an analyte to easily measurable signals are highly desired. A typical sensor is composed of three components to realize its functions: detection moieties, output domains and a platform to connect the two components. Bio-generated nano-biosensors are preferred because of the high surface-area-to-volume ratio and their bio-friendly nature. The E2 core of the pyruvate dehydrogenase complex from Bacillus stearothermophilus is a genetically-modifiable 60-mer protein nanocage of 27 nm diameter. In this talk, we will discuss our efforts in designing E2 nanocages as a platform for universal, high-sensitive, and reusable biosensor assembly. This was achieved by post-translational pairing of E2 nanocages with an antibody binding domain, a purification domain and a signal output domain at the required ratio. This post-translational modification strategy allowed universal antibody immobilization onto E2 nanocages while sensitive detection was achieved by the correct pairing with a signal-amplifying output domain.

Post-translational modifications of protein-nanocages as a universal biosensor platform

247th National Meeting (2014)

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