Quartz Crystal Microbalance with Dissipation (QCM-D) is a highly sensitive technique to measure the binding of a solute or suspended substance in solution to the surface of a test crystal which is periodically oscillated at high frequency by an alternating applied voltage. Any addition of mass to the crystal surface causes a proportional change in the frequency of oscillation of the crystal. Additionally, the visco-elastic properties of the adsorbed layer(s) can be inferred by monitoring the decay of oscillations and dissipation of energy into the layer � the higher the deformability of the layer, the stronger the dissipation and the faster the decay of oscillation. QCM-D technique has been used to monitor differential adsorption of two IgG2 monoclonal antibodies (mAb1 and mAb2) to multiple container closure and filter membrane surfaces and how their adsorption is differentially modulated by formulation excipients. Further, this methodology clearly demonstrates increased adsorption of mAb1 that has been subjected to agitation stress to the surfaces and that the magnitude of the change is dependent both on protein concentration and type of surface.

Surface adsorption characterization of IgG2 monoclonal antibodies by quartz crystal microbalance with dissipation (QCM-D)

247th National Meeting (2014)

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