Maple syrup generally contains a number of components as well as sucrose. We removed a large part of sucrose and prepared maple syrup extracts, MSE, MSx-high and MSx-low, rich in polyphenols at different levels. Animals were fed on diets with each of these polyphenol-rich extracts and the resulting effects of the diets on the bodily physiology were analyzed by genomics. Type II diabetes model mice (KK-Ay) were fed for 6 weeks on a standard AIN93G diet (control) with 0.1% MSE containing 6-8% polyphenol. Comparison of the MSE diet group with the control diet group in terms of blood biochemistry showed an increase in total ketone body and decreases in glycoalbumin as well as AST, ALT and LDH. Hepatic gene expression analysis revealed down-regulation of beta-oxidation associated genes (Acox1, Cptla, adipoR2) and up-regulation of keton synthesis gene (Hmgcs2) and inflammation-associated genes (Crp, Orm, and Saa) in the MSE-containing diet group. MSE ingestion improved insulin resistance, with the results that Irs2 and Gys2 were up-regulated while Foxo1, Pgela, Pck1, Gsk3b and Srebf1were down-regulated. These data suggest that KK-Ay mice in the MSE diet group were led to improvement of hyperglycemia by suppression of hepatic deposit and activation of insulin signaling. Next, C57BL/6J mice were fed for 8 weeks on a high-fat diet on the same diet with 0.06% MSx-low containing 1.6% polyphenol, with the result that MSx-low ingestion was effective for hepatic cholesterol synthesis and against inflammation. Then KK-Ay mice were fed for 6 weeks on AIN93G diet or on that with 0.05% MSx-high containing 16% polyphenol. This feeding also showed a body weight gain regulation and other desirable effects.

Analysis of physiological functions of maple syrup by nutrigenomics

247th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.