The design of hydrid liposome containing nanoparticles (NPs) has been attracted as therapeutics and therapeutics carriers. However, it was challenging to load NPs in the lipid bilayers due to the lack of hydrophobicity and difficulties of structural analysis of this system. In this paper, the hydrophobated NPs, C12AuNP, (1.17nm by SAXS) was synthesized and self-assembled in the bilayer of lipid-based nanodiscs. The nanodiscs are composed by different lipids (i.e., charge, chain length) of 1,2-dimyristoyl-sn-glycero-3-phosphocholine(DMPC)/ 1,2-dihexanoyl-sn-glycero-3-phosphocholine(DHPC)/ 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (DMPG). Also, by inducing the melting transition temperature of DMPC (23oC), the transformation of morphology from bicelles to ULV has confirmed by SAXS and light scattering. Both self-assembled structures successfully encapsulated the hydrophobated AuNPs in the lipid bilayers. The size and morphologies are determined by TEM, SAXS yielding a best-fit disc radius of 87nm and vesicular radius of 380nm at low and high temperatures, respectively. The findings also suggest a versatile way to understand the interaction between bilayers and NPs with different morphologies.

Self-assembly of unilamellar vesicles (ULV) with lipids and hydrophobated gold nanoparticles

247th National Meeting (2014)

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